Organotypic hippocampal slice cultures.
نویسندگان
چکیده
The hippocampus, a component of the limbic system, plays important roles in long-term memory and spatial navigation. Hippocampal neurons can modify the strength of their connections after brief periods of strong activation. This phenomenon, known as long-term potentiation (LTP) can last for hours or days and has become the best candidate mechanism for learning and memory. In addition, the well defined anatomy and connectivity of the hippocampus has made it a classical model system to study synaptic transmission and synaptic plasticity. As our understanding of the physiology of hippocampal synapses grew and molecular players became identified, a need to manipulate synaptic proteins became imperative. Organotypic hippocampal cultures offer the possibility for easy gene manipulation and precise pharmacological intervention but maintain synaptic organization that is critical to understanding synapse function in a more naturalistic context than routine culture dissociated neurons methods. Here we present a method to prepare and culture hippocampal slices that can be easily adapted to other brain regions. This method allows easy access to the slices for genetic manipulation using different approaches like viral infection or biolistics. In addition, slices can be easily recovered for biochemical assays, or transferred to microscopes for imaging or electrophysiological experiments.
منابع مشابه
Characterization of spontaneous network-driven synaptic activity in rat hippocampal slice cultures
A particular characteristic of the neonatal hippocampus is the presence of spontaneous network-driven oscillatory events, the so-called giant depolarizing potentials (GDPs). GDPs depend on the interplay between GABA and glutamate. Early in development, GABA, acting on GABAA receptors, depolarizes neuronal membranes via a Cl- efflux. Glutamate, via AMPA receptors, generates a positive feedback n...
متن کاملCharacterization of spontaneous network-driven synaptic activity in rat hippocampal slice cultures
A particular characteristic of the neonatal hippocampus is the presence of spontaneous network-driven oscillatory events, the so-called giant depolarizing potentials (GDPs). GDPs depend on the interplay between GABA and glutamate. Early in development, GABA, acting on GABAA receptors, depolarizes neuronal membranes via a Cl- efflux. Glutamate, via AMPA receptors, generates a positive feedback n...
متن کاملBorna disease virus replication in organotypic hippocampal slice cultures from rats results in selective damage of dentate granule cells.
In the hippocampus of Borna disease virus (BDV)-infected newborn rats, dentate granule cells undergo progressive cell death. BDV is noncytolytic, and the pathogenesis of this neurodevelopmental damage in the absence of immunopathology remains unclear. A suitable model system to study early events of the pathology is lacking. We show here that organotypic hippocampal slice cultures from newborn ...
متن کاملSpecific Aim 1: Determine how strain modulates the production of inflammatory cytokines and scar-associated chondroitin sulfate proteoglycans. Specific Aim 2: Determine whether strain-induced scarring within organotypic hippocampal slice cultures affects the recording stability
The specific aims have been slightly modified from the original proposal in that instead of using organotypic slice cultures to test our coatings and recording stability, we decided it was best to do this in vivo in the rat cortex. We generally believe that the in vivo cortical ‘test-bed’ is more rigorous than the organotypic slice cultures originally proposed. Additionally, it is more clinical...
متن کاملOnline Determination of Extracellular Glutathione in Organotypic Hippocampal Slice Cultures with a Mcirofluidic Device and Confocal Laser-induced Fluorescence Detection System
متن کامل
Axonal regeneration in hippocampal and spinal cord organotypic slice cultures
2. AIMSOFTHEPROJECT 32 2.1.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of visualized experiments : JoVE
دوره 48 شماره
صفحات -
تاریخ انتشار 2011